Expression of the Small Heat-Shock Genes During Drosophila Development - Comparison of the Accumulation of Hsp23 and Hsp27 Messenger-Rnas and Polypeptides

Pauli, D., Arrigo, A. P., Vazquez, J., Tonka, C. H., Tissieres, A. (January 1989) Expression of the Small Heat-Shock Genes During Drosophila Development - Comparison of the Accumulation of Hsp23 and Hsp27 Messenger-Rnas and Polypeptides. Genome, 31 (2). pp. 671-676. ISSN 0831-2796

URL: http://www.ncbi.nlm.nih.gov/pubmed/2517261
DOI: 10.1139/g89-126

Abstract

Seven heat shock genes are clustered within 15 kilobases of DNA at the Drosophila melanogaster chromosomal site 67B. They show a complex pattern of expression in the absence of external stress during normal development of this organism. In this paper, we quantitatively compare the abundance of the messenger RNAs for these seven genes at all major stages of Drosophila development and then focus on hsp23 and hsp27 for which available antibodies allow the comparison between the accumulation of the mRNAs and that of their corresponding polypeptides. Transcripts for both genes are maximally abundant in white prepupae. We observe that the amount of hsp23 message decreases more rapidly than that of hsp27 mRNA throughout the pupal period. The maximal abundance of the proteins occurs at the middle of the pupal stage, when their corresponding RNAs have almost completely disappeared. The peaks of expression of the proteins are also broader than those of their transcripts, indicating that the half-lives of the polypeptides are longer. These observations suggest that complex mechanisms regulate the expression of the small heat shock genes during Drosophila development.

Item Type: Paper
Subjects: organism description > animal > insect > Drosophila
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA
CSHL Authors:
Communities: CSHL labs
Depositing User: Gail Sherman
Date: January 1989
Date Deposited: 26 Jun 2017 18:46
Last Modified: 26 Jun 2017 18:46
Related URLs:
URI: http://repository.cshl.edu/id/eprint/34890

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving