Accurate and efficient RNA polymerase III transcription in a cell-free extract prepared from Ascaris suum embryos

Maroney, P. A., Hannon, G. J., Nilsen, T. W. (July 1989) Accurate and efficient RNA polymerase III transcription in a cell-free extract prepared from Ascaris suum embryos. Molecular and Biochemical Parasitology, 35 (3). pp. 277-283. ISSN 01666851

URL: http://www.ncbi.nlm.nih.gov/pubmed/2747746
DOI: 10.1016/0166-6851(89)90214-4

Abstract

High-speed supernatant (S100) extracts derived from homogenized Ascaris suum embryos efficiently transcribe added RNA polymerase III templates including cloned 5S rRNA genes of the filarial parasite Brugia malayi. Several criteria, including two-dimensional RNase T1 oligonucleotide fingerprint analysis, indicate that in vitro transcription is accurately initiated and terminated. © 1989.

Item Type: Paper
Uncontrolled Keywords: 5S rRNA Ascaris suum Brugia malayi In vitro transcription RNA polymerase III amanitin DNA directed RNA polymerase DNA directed RNA polymerase III DNA Directed RNA Polymerases RNA 5S animal article Ascaris biosynthesis Brugia cell free system comparative study female genetic transcription genetics molecular cloning nucleic acid hybridization regulator gene Xenopus Amanitins Cell-Free System Cloning, Molecular DNA-Directed RNA Polymerases RNA, Ribosomal, 5S Support, Non-U.S. Gov't Support, U.S. Gov't, Non-P.H.S. Support, U.S. Gov't, P.H.S. Terminator Regions (Genetics) Transcription, Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:
Communities: CSHL labs > Hannon lab
Depositing User: Gail Sherman
Date: July 1989
Date Deposited: 27 Jun 2017 16:20
Last Modified: 27 Jun 2017 16:20
Related URLs:
URI: http://repository.cshl.edu/id/eprint/34875

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