Maroney, P. A., Hannon, G. J., Nilsen, T. W. (July 1989) Accurate and efficient RNA polymerase III transcription in a cell-free extract prepared from Ascaris suum embryos. Molecular and Biochemical Parasitology, 35 (3). pp. 277-283. ISSN 01666851
Abstract
High-speed supernatant (S100) extracts derived from homogenized Ascaris suum embryos efficiently transcribe added RNA polymerase III templates including cloned 5S rRNA genes of the filarial parasite Brugia malayi. Several criteria, including two-dimensional RNase T1 oligonucleotide fingerprint analysis, indicate that in vitro transcription is accurately initiated and terminated. © 1989.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | 5S rRNA Ascaris suum Brugia malayi In vitro transcription RNA polymerase III amanitin DNA directed RNA polymerase DNA directed RNA polymerase III DNA Directed RNA Polymerases RNA 5S animal article Ascaris biosynthesis Brugia cell free system comparative study female genetic transcription genetics molecular cloning nucleic acid hybridization regulator gene Xenopus Amanitins Cell-Free System Cloning, Molecular DNA-Directed RNA Polymerases RNA, Ribosomal, 5S Support, Non-U.S. Gov't Support, U.S. Gov't, Non-P.H.S. Support, U.S. Gov't, P.H.S. Terminator Regions (Genetics) Transcription, Genetic |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > RNA polymerase bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor |
| CSHL Authors: | |
| Communities: | CSHL labs > Hannon lab |
| Depositing User: | Gail Sherman |
| Date: | July 1989 |
| Date Deposited: | 27 Jun 2017 16:20 |
| Last Modified: | 27 Jun 2017 16:20 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/34875 |
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