Inhibition of postsynaptic PKC or CaMKII blocks induction but not expression of LTP

Malinow, R., Schulman, H., Tsien, R. W. (August 1989) Inhibition of postsynaptic PKC or CaMKII blocks induction but not expression of LTP. Science, 245 (4920). pp. 862-6. ISSN 0036-8075

URL: http://www.ncbi.nlm.nih.gov/pubmed/2549638
DOI: 10.1126/science.2549638

Abstract

Long-term potentiation (LTP) of synaptic transmission is a widely studied cellular example of synaptic plasticity. However, the identity, localization, and interplay among the biochemical signals underlying LTP remain unclear. Intracellular microelectrodes have been used to record synaptic potentials and deliver protein kinase inhibitors to postsynaptic CA1 pyramidal cells. Induction of LTP is blocked by intracellular delivery of H-7, a general protein kinase inhibitor, or PKC(19-31), a selective protein kinase C (PKC) inhibitor, or CaMKII(273-302), a selective inhibitor of the multifunctional Ca2+-calmodulin-dependent protein kinase (CaMKII). After its establishment, LTP appears unresponsive to postsynaptic H-7, although it remains sensitive to externally applied H-7. Thus both postsynaptic PKC and CaMKII are required for the induction of LTP and a presynaptic protein kinase appears to be necessary for the expression of LTP.

Item Type: Paper
Uncontrolled Keywords: 1-(5-Isoquinolinesulfonyl)-2-Methylpiperazine Animals Ca(2+)-Calmodulin Dependent Protein Kinase Isoquinolines/pharmacology Piperazines/pharmacology Protein Kinase C/antagonists & inhibitors/*physiology Protein Kinase Inhibitors Protein Kinases/*physiology Rats Receptors, AMPA Receptors, Kainic Acid Receptors, Neurotransmitter/physiology Synapses/*physiology *Synaptic Transmission/drug effects
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > Long term potentiation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase > Protein kinase C
CSHL Authors:
Communities: CSHL labs > Malinow lab
Depositing User: Gail Sherman
Date: 25 August 1989
Date Deposited: 27 Jun 2017 16:11
Last Modified: 27 Jun 2017 16:11
Related URLs:
URI: http://repository.cshl.edu/id/eprint/34874

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