The cellular 107K protein that binds to adenovirus E1A also associates with the large T antigens of SV40 and JC virus

Dyson, N., Buchkovich, K., Whyte, P., Harlow, E. (July 1989) The cellular 107K protein that binds to adenovirus E1A also associates with the large T antigens of SV40 and JC virus. Cell, 58 (2). pp. 249-55. ISSN 0092-8674

URL: http://www.ncbi.nlm.nih.gov/pubmed/2546678
DOI: 10.1016/0092-8674(89)90839-8

Abstract

The association between the retinoblastoma protein (p105-RB) and either the large T antigen of SV40 or the E1A proteins of adenovirus is thought to be an important step in transformation by these viral oncogenes. E1A and large T antigen share a small region of amino acid homology that is necessary for high affinity binding with p105-RB. Mutations of this homology region were shown to reduce drastically the frequency of transformation mediated by the E1A or large T oncogenes. Previously, this small region in E1A was shown to be sufficient for interaction with a second cellular protein of 107,000 daltons (107K). Here we show that in human cells, the large T antigens of SV40 or JC virus also form complexes with 107K. Demonstration of complexes between 107K and the large T antigens of SV40 and JC virus suggests that these associations may represent another component of a common mechanism for transformation between adenoviruses and polyoma viruses.

Item Type: Paper
Uncontrolled Keywords: Adenovirus Early Proteins Antigens, Polyomavirus Transforming/*immunology Antigens, Viral, Tumor/*immunology Cell Line, Transformed Gene Expression Regulation Humans JC Virus/*immunology Oncogene Proteins, Viral/*metabolism Phosphoproteins/metabolism Polyomavirus/*immunology Proteins/immunology/*metabolism Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Retinoblastoma/genetics/metabolism Retinoblastoma Protein Simian virus 40/*immunology
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types
organism description > virus
CSHL Authors:
Communities: CSHL labs
Depositing User: Gail Sherman
Date: 28 July 1989
Date Deposited: 31 Jul 2017 18:34
Last Modified: 31 Jul 2017 18:34
Related URLs:
URI: http://repository.cshl.edu/id/eprint/34841

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