Noninvasive imaging of spontaneous retinoblastoma pathway-dependent tumors in mice

Vooijs, M., Jonkers, J., Lyons, S., Berns, A. (March 2002) Noninvasive imaging of spontaneous retinoblastoma pathway-dependent tumors in mice. Cancer Res, 62 (6). pp. 1862-7. ISSN 0008-5472 (Print)0008-5472 (Linking)

URL: https://www.ncbi.nlm.nih.gov/pubmed/11912166

Abstract

Identification of the critical pathways involved in tumorigenesis should ultimately lead to the design of better anticancer agents that target specific components of the disrupted pathways. Murine models of spontaneous cancer in which tumor formation is dependent on defined genetic alterations provide a powerful test system for evaluating the therapeutic efficacy of pathway-specific antineoplastics. We have generated a conditional mouse model for retinoblastoma-dependent sporadic cancer that permits noninvasive monitoring of pituitary tumor development in live animals via in vivo bioluminescence imaging of luciferase expression. We show that the high sensitivity of bioluminescence imaging can be used for noninvasive detection of luciferase expression in pituitary glands from tumor-free animals and for in vivo quantitation of tumor burden over a large dynamic range. This mouse model permits longitudinal monitoring of tumor onset, progression, and response to therapy and may be used effectively for testing cancer prevention and treatment strategies based on therapeutics that specifically target the retinoblastoma pathway.

Item Type: Paper
Uncontrolled Keywords: Animals Antibiotics, Antineoplastic/pharmacology Disease Models, Animal Doxorubicin/pharmacology Image Processing, Computer-Assisted/*methods Luciferases/genetics/metabolism Luminescent Measurements Mice Mice, Transgenic Pituitary Neoplasms/drug therapy/*pathology Pro-Opiomelanocortin/genetics Retinoblastoma/drug therapy/*pathology
Subjects: Investigative techniques and equipment > imaging
organism description > model organism
organism description > animal > mammal > rodent > mouse
diseases & disorders > cancer > cancer types > retinoblastoma
CSHL Authors:
Communities: CSHL labs > Lyons lab
Depositing User: Matt Covey
Date: 15 March 2002
Date Deposited: 12 May 2017 14:43
Last Modified: 12 May 2017 14:43
Related URLs:
URI: http://repository.cshl.edu/id/eprint/34729

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