Imaging mouse cancer models in vivo using reporter transgenes

Lyons, S. K., Patrick, P. S., Brindle, K. M. (August 2013) Imaging mouse cancer models in vivo using reporter transgenes. Cold Spring Harb Protoc, 2013 (8). pp. 685-99. ISSN 1559-6095 (Electronic)1559-6095 (Linking)

URL: https://www.ncbi.nlm.nih.gov/pubmed/23906907
DOI: 10.1101/pdb.top069864

Abstract

Imaging mouse models of cancer with reporter transgenes has become a relatively common experimental approach in the laboratory, which allows noninvasive and longitudinal investigation of diverse aspects of tumor biology in vivo. Our goal here is to outline briefly the principles of the relevant imaging modalities, emphasizing particularly their strengths and weaknesses and what the researcher can expect in a practical sense from each of these techniques. Furthermore, we discuss how relatively subtle modifications in the way reporter transgene expression is regulated in the cell underpin the ability of reporter transgenes as a whole to provide readouts on such varied aspects of tumor biology in vivo.

Item Type: Paper
Uncontrolled Keywords: Animals Disease Models, Animal *Genes, Reporter Image Processing, Computer-Assisted/methods Neoplasms/*pathology Optical Imaging/*methods *Transgenes Whole Body Imaging/*methods
Subjects: diseases & disorders > cancer
Investigative techniques and equipment > imaging
organism description > model organism
organism description > animal > mammal > rodent > mouse
CSHL Authors:
Communities: CSHL labs > Lyons lab
Depositing User: Matt Covey
Date: 1 August 2013
Date Deposited: 12 May 2017 16:09
Last Modified: 12 May 2017 16:09
Related URLs:
URI: http://repository.cshl.edu/id/eprint/34721

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