The generation of a conditional reporter that enables bioluminescence imaging of Cre/loxP-dependent tumorigenesis in mice

Lyons, S. K., Meuwissen, R., Krimpenfort, P., Berns, A. (November 2003) The generation of a conditional reporter that enables bioluminescence imaging of Cre/loxP-dependent tumorigenesis in mice. Cancer Res, 63 (21). pp. 7042-6. ISSN 0008-5472 (Print)0008-5472 (Linking)

URL: https://www.ncbi.nlm.nih.gov/pubmed/14612492

Abstract

The ability to noninvasively quantitate tumor burden from conditional (Cre/loxP-dependent) mouse cancer models would greatly increase their range of useful applications. We now report the generation of a reporter mouse that enables visualization of spontaneous tumor development from pre-existing conditional mouse tumor models via in vivo bioluminescence imaging. We demonstrate that bioluminescence can be "switched-on" in a Cre-dependent manner in every organ analyzed, and that this gives rise to between a 4 and 6-log increase in light emission per mg of wet tissue weight. Furthermore, we highlight the utility of this reporter by showing that it can be used as a sensitive means to measure spontaneous Kras2(v12)-induced lung tumorigenesis in a pre-existing mouse model of non-small cell lung cancer. Taken together, our results suggest that this reporter may be combined with a wide-range of other Cre/loxP tumor mouse models, irrespective of their tissue specificity and render them immediately amenable to longitudinal monitoring of tumor growth and therapeutic response with a noninvasive in vivo imaging approach.

Item Type: Paper
Uncontrolled Keywords: Animals Carcinoma, Non-Small-Cell Lung/genetics/metabolism/*pathology Disease Models, Animal Genetic Vectors/genetics Integrases/*genetics/metabolism Luciferases/*genetics/metabolism Luminescent Measurements Luminescent Proteins/*genetics/metabolism Lung Neoplasms/genetics/metabolism/*pathology Mice Mice, Transgenic Viral Proteins/*genetics/metabolism
Subjects: Investigative techniques and equipment > imaging
diseases & disorders > cancer > cancer types > lung cancer
organism description > animal > mammal > rodent > mouse
CSHL Authors:
Communities: CSHL labs > Lyons lab
Depositing User: Matt Covey
Date: 1 November 2003
Date Deposited: 12 May 2017 15:52
Last Modified: 12 May 2017 15:52
Related URLs:
URI: http://repository.cshl.edu/id/eprint/34720

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