Single-minded CRISPR screening

Lanning, B. R., Vakoc, C. R. (April 2017) Single-minded CRISPR screening. Nat Biotechnol, 35 (4). pp. 339-340. ISSN 1087-0156

URL: https://www.ncbi.nlm.nih.gov/pubmed/28398325
DOI: 10.1038/nbt.3849

Abstract

ooled CRISPR knockout screening is a powerful technique for interrogating the biological function of genes in a non-biased manner. It can, however, only identify genes that influence cellular growth or viability phenotypes or alter the expression of a limited number of specific markers detected using antibodies or fluorescent protein reporters1, 2. Four recent studies published in Cell3, 4, 5 and Nature Methods6 now describe methods (independently termed Perturb-Seq3, 4, CRISP-seq5, or CROP-seq6) that combine pooled CRISPR screening with single-cell RNA-seq analysis, thereby linking each gene knockout with its effect on the expression level of any transcribed gene. These methods expand the resolving power of genetic screens in mammalian cells by not only increasing the number of potential readouts, but also enabling the investigation of the cell-to-cell differences in the effects of genetic knockout. They are therefore likely to have far-reaching applications for the elucidation of biological circuits.

Item Type: Paper
Subjects: Investigative techniques and equipment > CRISPR-Cas9
CSHL Authors:
Communities: CSHL labs > Vakoc lab
CSHL Cancer Center Program > Gene Regulation and Cell Proliferation
Depositing User: Matt Covey
Date: 11 April 2017
Date Deposited: 20 Apr 2017 15:52
Last Modified: 12 Jun 2018 19:31
Related URLs:
URI: http://repository.cshl.edu/id/eprint/34577

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving