Benign mesenchymal stromal cells in human sarcomas

Morozov, A., Downey, R. J., Healey, J., Moreira, A. L., Lou, E., Franceschino, A., Dogan, Y., Leung, R., Edgar, M., LaQuaglia, M., Maki, R. G., Moore, M. A. (December 2010) Benign mesenchymal stromal cells in human sarcomas. Clin Cancer Res, 16 (23). pp. 5630-40. ISSN 1078-0432 (Print)1078-0432 (Linking)

URL: https://www.ncbi.nlm.nih.gov/pubmed/21138865
DOI: 10.1158/1078-0432.CCR-09-2886

Abstract

PURPOSE: Recent evidence suggests that at least some sarcomas arise through aberrant differentiation of mesenchymal stromal cells (MSCs), but MSCs have never been isolated directly from human sarcoma specimens. EXPERIMENTAL DESIGN: We examined human sarcoma cell lines and primary adherent cultures derived from human sarcoma surgical samples for features of MSCs. We further characterized primary cultures as either benign or malignant by the presence of tumor-defining genetic lesions and tumor formation in immunocompromised mice. RESULTS: We show that a dedifferentiated liposarcoma cell line DDLS8817 posesses fat, bone, and cartilage trilineage differentiation potential characteristic of MSCs. Primary sarcoma cultures have the morphology, surface immunophenotype, and differentiation potential characteristic of MSCs. Surprisingly, many of these cultures are benign, as they do not form tumors in mice and lack sarcoma-defining genetic lesions. Consistent with the recently proposed pericyte origin of MSCs in normal human tissues, sarcoma-derived benign MSCs (SDBMSCs) express markers of pericytes and cooperate with endothelial cells in tube formation assays. In human sarcoma specimens, a subset of CD146-positive microvascular pericytes expresses CD105, an MSC marker, whereas malignant cells largely do not. In an in vitro coculture model, SDBMSCs as well as normal human pericytes markedly stimulate the growth of sarcoma cell lines. CONCLUSIONS: SDBMSCs/pericytes represent a previously undescribed stromal cell type in sarcoma that may contribute to tumor formation.

Item Type: Paper
Uncontrolled Keywords: Animals Cell Differentiation/physiology Cell Line, Tumor Cell Separation/methods Drug Evaluation, Preclinical/methods Female Humans Interleukin Receptor Common gamma Subunit/genetics Mesenchymal Stromal Cells/*pathology/*physiology Mice Mice, Inbred NOD Mice, SCID Mice, Transgenic Sarcoma/*pathology Stromal Cells/*pathology/*physiology Transplantation, Heterologous
Subjects: Investigative techniques and equipment > cell culture
diseases & disorders > cancer > cancer types > sarcoma
CSHL Authors:
Communities: CSHL labs > Maki lab
Depositing User: Matt Covey
Date: 1 December 2010
Date Deposited: 21 Oct 2016 20:10
Last Modified: 21 Oct 2016 20:10
PMCID: PMC3820159
Related URLs:
URI: http://repository.cshl.edu/id/eprint/33712

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving