Mammary Tumor-Associated RNAs Impact Tumor Cell Proliferation, Invasion, and Migration

Diermeier, Sarah D, Chang, Kung-Chi, Freier, Susan M, Song, Junyan, El Demerdash, Osama, Krasnitz, Alexander, Rigo, Frank, Bennett, C.  Frank, Spector, David L (2016) Mammary Tumor-Associated RNAs Impact Tumor Cell Proliferation, Invasion, and Migration. Cell Reports, 17 (1). pp. 261-274. ISSN 2211-1247

URL: https://www.ncbi.nlm.nih.gov/pubmed/27681436
DOI: 10.1016/j.celrep.2016.08.081

Abstract

Long non-coding RNAs (lncRNAs) represent the largest and most diverse class of non-coding RNAs, comprising almost 16,000 currently annotated transcripts in human and 10,000 in mouse. Here, we investigated the role of lncRNAs in mammary tumors by performing RNA-seq on tumor sections and organoids derived from MMTV-PyMT and MMTV-Neu-NDL mice. We identified several hundred lncRNAs that were overexpressed compared to normal mammary epithelium. Among these potentially oncogenic lncRNAs we prioritized a subset as Mammary Tumor Associated RNAs (MaTARs) and determined their human counterparts, hMaTARs. To functionally validate the role of MaTARs, we performed antisense knockdown and observed reduced cell proliferation, invasion, and/or organoid branching in a cancer-specific context. Assessing the expression of hMaTARs in human breast tumors revealed that 19 hMaTARs are significantly upregulated and many of these correlate with breast cancer subtype and/or hormone receptor status, indicating potential clinical relevance.

Item Type: Paper
Subjects: diseases & disorders > cancer > cancer types > breast cancer
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell migration
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell proliferation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > long non-coding RNA
organs, tissues, organelles, cell types and functions > tissues types and functions > mammary gland
CSHL Authors:
Communities: CSHL labs > Krasnitz lab
CSHL labs > Spector lab
Highlight: Spector, David L.
Depositing User: Matt Covey
Date Deposited: 28 Sep 2016 19:38
Last Modified: 07 Sep 2017 14:05
PMCID: PMC5079290
Related URLs:
Dataset ID:
URI: http://repository.cshl.edu/id/eprint/33551

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