Constitutive turnover of histone H2A.Z at yeast promoters requires the preinitiation complex

Tramantano, M., Sun, L., Au, C., Labuz, D., Liu, Z., Chou, M., Shen, C., Luk, E. (2016) Constitutive turnover of histone H2A.Z at yeast promoters requires the preinitiation complex. Elife, 5. ISSN 2050-084X (Electronic)2050-084X (Linking)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/27438412
DOI: 10.7554/eLife.14243

Abstract

The assembly of the preinitiation complex (PIC) occurs upstream of the +1 nucleosome which, in yeast, obstructs the transcription start site and is frequently assembled with the histone variant H2A.Z. To understand the contribution of the transcription machinery in the disassembly of the +1 H2A.Z nucleosome, conditional mutants were used to block PIC assembly. A quantitative ChIP-seq approach, which allows detection of global occupancy change, was employed to measure H2A.Z occupancy. Blocking PIC assembly resulted in promoter-specific H2A.Z accumulation, indicating that the PIC is required to evict H2A.Z. By contrast, H2A.Z eviction was unaffected upon depletion of INO80, a remodeler previously reported to displace nucleosomal H2A.Z. Robust PIC-dependent H2A.Z eviction was observed at active and infrequently transcribed genes, indicating that constitutive H2A.Z turnover is a general phenomenon. Finally, sites with strong H2A.Z turnover precisely mark transcript starts, providing a new metric for identifying cryptic and alternative sites of initiation.

Item Type: Paper
Uncontrolled Keywords: ChIP-seq S. cerevisiae biochemistry chromatin chromatin remodelers chromosomes genes histone variants transcription factors
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Chromatin dynamics
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > histone
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Stillman lab
CSHL labs > Vakoc lab
Depositing User: Matt Covey
Date Deposited: 22 Sep 2016 20:31
Last Modified: 03 Nov 2017 18:41
PMCID: PMC4995100
Related URLs:
URI: http://repository.cshl.edu/id/eprint/33540

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