Genetic analysis of adenovirus type 2. VIII. Physical locations of temperature-sensitive mutations

Hassell, J. A., Weber, J. (December 1978) Genetic analysis of adenovirus type 2. VIII. Physical locations of temperature-sensitive mutations. J Virol, 28 (3). pp. 671-8. ISSN 0022-538X (Print)0022-538X (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/731791

Abstract

Temperature-sensitive mutations of human adenoviruses can be physically located on the viral genome by determining the DNA structures of recombinants formed in genetic crosses between different members of the same subgroup. We have analyzed the DNA structures of many interserotypic recombinants formed in crosses between temperature-sensitive (ts) mutants of adenovirus type 2 and adenovirus type 5 with the restriction endonucleases BamHI, EcoRI, HindIII, and and Sma I. In this way, we have mapped the physical coordinates of adenovirus type 2 (Ad2) ts1, Ad2 ts3, Ad2 ts4, and Ad2 ts48, and refined the mapping of Ad5 ts1.

Item Type: Paper
Uncontrolled Keywords: Adenoviruses, Human/*genetics DNA Restriction Enzymes DNA, Viral/analysis/*genetics *Genes, Viral Mutation Recombination, Genetic Temperature
Subjects: organism description > virus > adenovirus
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > restriction enzyme
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: December 1978
Date Deposited: 24 Aug 2016 16:05
Last Modified: 24 Aug 2016 16:05
PMCID: PMC525790
Related URLs:
URI: http://repository.cshl.edu/id/eprint/33344

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