Toussaint, A., Lefebvre, N., Scott, J. R., Cowan, J. A., de Bruijn, F., Bukhari, A. I. (August 1978) Relationships between temperate phages Mu and P1. Virology, 89 (1). pp. 146-61. ISSN 0042-6822 (Print)0042-6822 (Linking)
Abstract
The temperate phages Mu and P1, although extremely different, share a short region of homology which includes the G region of Mu (termed the C region in P1). We show that the region of homology between Mu and P1 contains gene(s) that code for proteins responsible for the phages' host range specificity. Mu-P1 hybrids with the plaque morphology and immunity of Mu have been isolated by crossing Mu with two different P1 derivatives; these hybrids may acquire the host range of the P1 mutant used in the cross. Mu am+ recombinants were isolated after superinfection of a P1 lysogen with Mu Sam or Mu Uam mutants, showing that these two Mu genes are located in the region of homology between P1 and Mu. P1 am+recombinants are found after superinfection of a Mu lysogen with Plam33, am72, and am66, indicating that these P1 alleles are all located in the region of homology with Mu. From these crosses, P1-immune hybrids have been isolated with both P1 and Mu host range. There is partial serological cross-reactivity between P1 and Mu, and hybrid phages are in most cases inactivated by antiserum at a rate comparable to either parent or intermediate between them.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Coliphages/*genetics/growth & development DNA, Viral *Genes, Viral Genetic Linkage Lysogeny Mutation Nucleic Acid Hybridization Recombination, Genetic Viral Plaque Assay |
| Subjects: | organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | August 1978 |
| Date Deposited: | 23 Aug 2016 20:51 |
| Last Modified: | 23 Aug 2016 20:51 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/33340 |
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