Relationships between temperate phages Mu and P1

Toussaint, A., Lefebvre, N., Scott, J. R., Cowan, J. A., de Bruijn, F., Bukhari, A. I. (1978) Relationships between temperate phages Mu and P1. Virology, 89 (1). pp. 146-61. ISSN 0042-6822 (Print)0042-6822 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/685175
DOI: 10.1016/0042-6822(78)90048-X

Abstract

The temperate phages Mu and P1, although extremely different, share a short region of homology which includes the G region of Mu (termed the C region in P1). We show that the region of homology between Mu and P1 contains gene(s) that code for proteins responsible for the phages' host range specificity. Mu-P1 hybrids with the plaque morphology and immunity of Mu have been isolated by crossing Mu with two different P1 derivatives; these hybrids may acquire the host range of the P1 mutant used in the cross. Mu am+ recombinants were isolated after superinfection of a P1 lysogen with Mu Sam or Mu Uam mutants, showing that these two Mu genes are located in the region of homology between P1 and Mu. P1 am+recombinants are found after superinfection of a Mu lysogen with Plam33, am72, and am66, indicating that these P1 alleles are all located in the region of homology with Mu. From these crosses, P1-immune hybrids have been isolated with both P1 and Mu host range. There is partial serological cross-reactivity between P1 and Mu, and hybrid phages are in most cases inactivated by antiserum at a rate comparable to either parent or intermediate between them.

Item Type: Paper
Uncontrolled Keywords: Coliphages/*genetics/growth & development DNA, Viral *Genes, Viral Genetic Linkage Lysogeny Mutation Nucleic Acid Hybridization Recombination, Genetic Viral Plaque Assay
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date Deposited: 23 Aug 2016 20:51
Last Modified: 23 Aug 2016 20:51
Related URLs:
URI: http://repository.cshl.edu/id/eprint/33340

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