Studies on intercellular LETS glycoprotein matrices

Chen, L. B., Murray, A., Segal, R. A., Bushnell, A., Walsh, M. L. (1978) Studies on intercellular LETS glycoprotein matrices. Cell, 14 (2). pp. 377-91. ISSN 0092-8674 (Print)0092-8674 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/667946
DOI: 10.1016/0092-8674(78)90123-X

Abstract

Intercellular matrices secreted by chick embryo fibroblasts in culture were studied by scanning electron microscopy. Cell-cell contact is a prerequisite for the expression of such matrices. The smallest fiber detected by transmission electron microscopy is 5--10 nm in diameter. These matrix fibers tend to cluster to form bundles. Immunofluorescence and immunoferritin procedures reveal that LETS protein is one of the components of the matrices. The matrices are isolated from other cellular organelles by detergent treatment. More than 90% of the proteins in cell-free matrices are LETS protein, suggesting that the matrices are probably made of only one component--LETS protein. Since the solubilization of matrices requires beta-mercaptoethanol, LETS protein matrices may be the first known polymer system in nature to use disulfide linkage as an intermolecular polymerization vehicle. Collagen does not appear to be involved in such matrices. The LETS protein matrix supports the morphological conversion of rounded cells into spindle-shaped, and also promotes myoblast fusion. It does not, however, exert an effect upon cell growth, the rate of glucose uptake or protease production.

Item Type: Paper
Uncontrolled Keywords: *Cell Count Cell Division Cell Fusion Cell-Free System Cells, Cultured Glucose/metabolism Glycoproteins/*metabolism Membrane Proteins/*metabolism Mercaptoethanol/pharmacology Microscopy, Electron, Scanning Peptide Hydrolases/biosynthesis
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > fibroblasts

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > matrix protein
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: June 1978
Date Deposited: 25 Aug 2016 12:19
Last Modified: 25 Aug 2016 12:19
Related URLs:
URI: http://repository.cshl.edu/id/eprint/33337

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