Adjacent insertion sequences IS2 and IS5 in bacteriophage Mu mutants and an IS5 in a lambda darg bacteriophage

Chow, L. T., Broker, T. R. (March 1978) Adjacent insertion sequences IS2 and IS5 in bacteriophage Mu mutants and an IS5 in a lambda darg bacteriophage. J Bacteriol, 133 (3). pp. 1427-36. ISSN 0021-9193 (Print)0021-9193 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/641012

Abstract

Using electron microscopic heteroduplex analysis, we have demonstrated that an insertion found in a Mu prophage and in some infectious. Mu deletion-substitution mutants derived from it consists of bacterial insertion sequence IS2 linked directly to IS5. Other infectious Mu mutants derived from the same lysogen have only IS5 or a portion of IS2. In addition, we have found that an independent insertion in a transducing phage, lambda 13 dargB2, is IS5. The ends of IS5 are short, inverted duplications of each other. These observations support the notion that the DNA insertion previously designated IS5 on the basis of a single example in lambda KH100 is a bona fide bacterial insertion sequence.

Item Type: Paper
Uncontrolled Keywords: Coliphages/*genetics *DNA, Bacterial *Genes, Viral Lysogeny Mutation Nucleic Acid Hybridization Transduction, Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > insertion/deletion
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: March 1978
Date Deposited: 25 Aug 2016 11:48
Last Modified: 25 Aug 2016 11:48
PMCID: PMC222181
Related URLs:
URI: http://repository.cshl.edu/id/eprint/33335

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