Kamp, D., Kahmann, R., Zipser, D., Broker, T. R., Chow, L. T. (February 1978) Inversion of the G DNA segment of phage Mu controls phage infectivity. Nature, 271 (5645). pp. 577-80. ISSN 0028-0836 (Print)0028-0836 (Linking)
Abstract
The 3,000 base pair-long G segment of temperate coliphage Mu DNA has been shown to invert spontaneously with high frequency1,2. Inversion, which is independent of the rec system of the host3, results in Mu phage with the G segment in either orientation. Nearly equal numbers of both G orientations are observed in a lysogen or when a lysate is obtained by induction of a lysogen. When phage is grown by a series of infections one and the same G orientation prevails4. One attractive explanation for these observations implicates inversion of the G segment in the control of phage or host functions. We report here experiments which show that the orientation of the G segment determines infectivity of Mu phage particles and that G inversion is mediated by a phage-specific enzyme system.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Base Sequence Coliphages/*genetics/pathogenicity DNA, Viral/*physiology Viral Plaque Assay |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage organism description > virus |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | 9 February 1978 |
| Date Deposited: | 24 Aug 2016 20:13 |
| Last Modified: | 24 Aug 2016 20:13 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/33332 |
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