Isolation and analysis of recombinant DNA molecules containing yeast DNA

Petes, T. D., Broach, J. R., Wensink, P. C., Hereford, L. M., Fink, G. R., Botstein, D. (1978) Isolation and analysis of recombinant DNA molecules containing yeast DNA. Gene, 4 (1). pp. 37-49. ISSN 0378-1119 (Print)0378-1119 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/365691
DOI: 10.1016/0378-1119(78)90013-6

Abstract

2500 recombinant plasmids containing insertions of yeast nuclear DNA have been cloned in Escherichia coli. It can be calculated that about 85% of the yeast genome is represented in this collection. The clones have been characterized by hybridization to purified RNA species. Of the 2000 clones examined, 75 contain insertions of yeast ribosomal DNA, 201 contain insertions of yeast tRNA genes, and 26 contain DNA sequences that are complementary to abundant mRNA species.

Item Type: Paper
Uncontrolled Keywords: DNA/*genetics DNA, Bacterial/genetics DNA, Recombinant/*isolation & purification Escherichia coli/genetics Nucleic Acid Hybridization Plasmids RNA/genetics RNA, Messenger/genetics RNA, Ribosomal/genetics RNA, Transfer/genetics Saccharomyces cerevisiae/*genetics
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > recombination
organism description > yeast
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: September 1978
Date Deposited: 25 Aug 2016 13:50
Last Modified: 25 Aug 2016 13:50
Related URLs:
URI: http://repository.cshl.edu/id/eprint/33325

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