Insertion of a transposon for chloramphenicol resistance into bacteriophage Mu

Bukhari, A. I., Froshauer, S. (July 1978) Insertion of a transposon for chloramphenicol resistance into bacteriophage Mu. Gene, 3 (4). pp. 303-14. ISSN 0378-1119 (Print)0378-1119 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/365685
DOI: 10.1016/0378-1119(78)90040-9

Abstract

We have isolated mutants of bacteriophage Mu carrying the X mutations caused by the insertion of cam (Tn9), a transposon for chloramphenicol resistance. The Mu X cam mutants were obtained by selecting for heat-resistant survivors of a Mucts62, P1cam dilysogen. Like the previously described X mutants, Mu X cam mutants are defective prophages which can be excised from the host DNA at a frequency of 10(-5) to 10(-7) per cell. Tn9 insertions in Mu X cam mutants are located within 5000 base pairs of the left end of Mu DNA in a region that controls early replication functions of Mu. There is one EcoRI cleavage site in Tn9. The Tn9 transposon itself can be excised precisely from the Mu X cam mutants to generate wild type Mu. In most Mu X cam mutants, precise excision of Tn9 occurs at a low frequency (10(-6) per cell), whereas in some, the frequency is higher (10(-4) per cell). Mu X cam prophages can replicate after induction with the help of wild type Mu. The lysates containing Mu X cam particles, however, fail to transduce chloramphenicol resistance at a high frequency; Mu X cam mutants apparently have a cis dominant defect in integration.

Item Type: Paper
Uncontrolled Keywords: *Chloramphenicol Coliphages/*genetics DNA, Bacterial/genetics *DNA, Recombinant DNA, Viral/genetics *Drug Resistance, Microbial Escherichia coli/genetics Mutation *R Factors Transduction, Genetic
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage

organism description > bacteria > escherichia coli
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transposons
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: July 1978
Date Deposited: 25 Aug 2016 12:52
Last Modified: 25 Aug 2016 12:52
Related URLs:
URI: http://repository.cshl.edu/id/eprint/33322

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