Bigelis, R., Burridge, K. (May 1978) The immunological detection of yeast nonsense termination fragments on sodium dodecylsulfate-polyacrylamide gels. Biochem Biophys Res Commun, 82 (1). pp. 322-7. ISSN 0006-291X (Print)0006-291X (Linking)
Abstract
A sensitive immunological technique is described that detects proteins in sodium dodecylsulfate gels of crude cell extracts. The method is based on the binding of 125I-protein A to gels that have been incubated with antibody to a specific protein. Using antibody to the yeast HIS4 protein, single polypeptides can be detected in mutant and wild-type extracts. The size of these polypeptides correlates both with the type of mutation and with its location in the HIS4,m region.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Electrophoresis, Polyacrylamide Gel *Fungal Proteins/biosynthesis Immunoassay Molecular Weight *Mutation Peptides/*analysis Saccharomyces cerevisiae/*genetics |
| Subjects: | Investigative techniques and equipment > assays > gel electrophoresis organism description > yeast |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | 15 May 1978 |
| Date Deposited: | 29 Aug 2016 16:06 |
| Last Modified: | 29 Aug 2016 16:06 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/33315 |
Actions (login required)
 |
Administrator's edit/view item |
