The immunological detection of yeast nonsense termination fragments on sodium dodecylsulfate-polyacrylamide gels

Bigelis, R., Burridge, K. (May 1978) The immunological detection of yeast nonsense termination fragments on sodium dodecylsulfate-polyacrylamide gels. Biochem Biophys Res Commun, 82 (1). pp. 322-7. ISSN 0006-291X (Print)0006-291X (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/352360
DOI: 10.1016/0006-291X(78)90612-5

Abstract

A sensitive immunological technique is described that detects proteins in sodium dodecylsulfate gels of crude cell extracts. The method is based on the binding of 125I-protein A to gels that have been incubated with antibody to a specific protein. Using antibody to the yeast HIS4 protein, single polypeptides can be detected in mutant and wild-type extracts. The size of these polypeptides correlates both with the type of mutation and with its location in the HIS4,m region.

Item Type: Paper
Uncontrolled Keywords: Electrophoresis, Polyacrylamide Gel *Fungal Proteins/biosynthesis Immunoassay Molecular Weight *Mutation Peptides/*analysis Saccharomyces cerevisiae/*genetics
Subjects: Investigative techniques and equipment > assays > gel electrophoresis
organism description > yeast
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: 15 May 1978
Date Deposited: 29 Aug 2016 16:06
Last Modified: 29 Aug 2016 16:06
Related URLs:
URI: http://repository.cshl.edu/id/eprint/33315

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