Characterization of the intermediate (10 nm) filaments of cultured cells using an autoimmune rabbit antiserum

Gordon, W. E., Bushnell, A., Burridge, K. (February 1978) Characterization of the intermediate (10 nm) filaments of cultured cells using an autoimmune rabbit antiserum. Cell, 13 (2). pp. 249-61. ISSN 0092-8674 (Print)0092-8674 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/342115
DOI: 10.1016/0092-8674(78)90194-0

Abstract

An antiserum has been found in a nonimmunized rabbit which reacts strongly with a system of filaments in various fibroblasts, epithelial cells, macrophages and neuroblastoma. These filaments are distinct from the actin microfilament bundles visualized by an antibody against actin, and they are not affected by brief treatment with cytochalasin B. The pattern of these filaments somewhat resembles that described for microtubules, but the filaments could be clearly distinguished from microtubules by a comparison of their respective immunofluorescent patterns during cell division. In response to the drugs colcemid and vinblastine, the filaments reacting with this preimmune serum condense to form a compact perinuclear coil of fibers, a distribution and behavior in agreement with that previously described for the 10 nm or intermediate filaments studied by electron microscopy. Further evidence supporting our conclusion that this antiserum reacts with intermediate filaments is provided by a comparison of electron micrographs and the immunofluorescent patterns from parallel cell cultures. To identify the antigens reacting with this antiserum we have used the new technique of immuno-autoradiography on SDS gels of whole cell extracts. Two reactive polypeptide chains have been identified with apparent molecular weights of 56,000 and 30,000 daltons.

Item Type: Paper
Uncontrolled Keywords: Animals Autoradiography Cell Line Colchicine/pharmacology Cytochalasin B/pharmacology Cytoplasm/*analysis Cytoskeleton/*analysis/drug effects/immunology Fluorescent Antibody Technique Immune Sera Rabbits Tubulin/immunology Vinblastine/pharmacology
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > actin
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: February 1978
Date Deposited: 12 Dec 2016 20:44
Last Modified: 12 Dec 2016 20:44
Related URLs:
URI: http://repository.cshl.edu/id/eprint/33311

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