Another chromosomal assignment for a simian virus 40 integration site in human cells

Kucherlapati, R., Hwang, S. P., Shimizu, N., McDougall, J. K., Botchan, M. R. (September 1978) Another chromosomal assignment for a simian virus 40 integration site in human cells. Proc Natl Acad Sci U S A, 75 (9). pp. 4460-4. ISSN 0027-8424 (Print)0027-8424 (Linking)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/212753

Abstract

Somatic cell hybrids derived from fusion of GM637, a human cell line transformed by simian virus 40, and mouse B82 cells were examined for simian virus 40 T antigen, V antigen, and viral DNA. All hybrid cell lines that contained viral DNA were T-antigen positive. Cells that did not have viral DNA were T-antigen negative. We determined that there is a single viral insertion in these hybrid cells. Correlation of T-antigen expression and viral DNA with the partial complements of the human genome retained in the hybrids shwed that the inserted viral genome is in human chromosome 8. The integrated viral DNA is stable; free viral DNA found in GM637 does not insert at other potential sites in the human genome.

Item Type: Paper
Uncontrolled Keywords: Antigens, Viral/analysis Cell Line *Chromosomes, Human, 6-12 and X DNA, Viral/analysis/*metabolism Humans Hybrid Cells/microbiology Simian virus 40/*genetics/immunology Virus Replication
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome

organism description > virus > SV40
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: September 1978
Date Deposited: 13 Dec 2016 17:07
Last Modified: 09 Nov 2017 21:02
PMCID: PMC336135
Related URLs:
URI: http://repository.cshl.edu/id/eprint/33303

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