Characterization and sequence analysis of a recombination site in the hybrid virus Ad2+ND

Zain, B. S., Roberts, R. J. (March 1978) Characterization and sequence analysis of a recombination site in the hybrid virus Ad2+ND. J Mol Biol, 120 (1). pp. 13-31. ISSN 0022-2836 (Print)0022-2836 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/205651
DOI: 10.1016/0022-2836(78)90293-0

Abstract

Restriction endonuclease mapping of an adenovirus-simian virus 40 hybrid virus and adenovirus-2 DNA allowed the characterization of fragments of Ad2+ND1† which contain the two junctions between simian virus (SV40) sequences and adenovirus-2 sequences. The corresponding fragments of Ad2++ DNA were also characterized. One fragment of Ad2+ND1 containing a recombination site, and the corresponding fragment of Ad2++ were analyzed by direct DNA sequence analysis. Comparison of nucleotide sequences in Ad2++, Ad2+ ND1, and SV40 DNAs precisely localized those sequences involved in the final recombination event which produced the stable hybrid virus Ad2+ND1. No sequence homology was detected between the two parent DNAs.

Item Type: Paper
Uncontrolled Keywords: Adenoviridae Base Sequence Chromosome Mapping DNA Restriction Enzymes DNA, Viral/*analysis/genetics Electrophoresis Nucleic Acid Hybridization *Recombination, Genetic Simian virus 40
Subjects: organism description > virus > adenovirus
CSHL Authors:
Communities: CSHL labs > Roberts lab
Depositing User: Matt Covey
Date: 25 March 1978
Date Deposited: 13 Dec 2016 15:13
Last Modified: 13 Dec 2016 15:13
Related URLs:
URI: http://repository.cshl.edu/id/eprint/33299

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving