Isolation and characterization of T antigen-negative revertants from a line of transformed rat cells containing one copy of the SV40 genome

Steinberg, B., Pollack, R., Topp, W., Botchan, M. (January 1978) Isolation and characterization of T antigen-negative revertants from a line of transformed rat cells containing one copy of the SV40 genome. Cell, 13 (1). pp. 19-32. ISSN 0092-8674 (Print)0092-8674 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/202400
DOI: 10.1016/0092-8674(78)90134-4

Abstract

Negative selection with FUdR produced revertants from the transformed rat line 14B, which contains one insertion of the SV40 viral genome (Botchan, Topp and Sambrook, 1976). 14B contains nuclear T antigen, grows to a high density, grows in low serum and is anchorage-independent. The revertants fall into three classes with regard to viral DNA sequences: the SV40 DNA is retained; the SV40 DNA is retained but has undergone a deletion; and the SV40 DNA is lost, generating a cured cell. This heterogeneity is not a result of long-term passage. The revertants arise with a frequency of one in 8.4 X 10(5) cells after as few as 12 passages. All three classes of revertants are T antigen-negative, density-sensitive, more serum sensitive than 14B and anchorage-dependent. These data argue for a direct role of the functioning viral genome in the maintenance of the transformed state, and that with 14B, the phenotypes of transformation are not virus gene dosage-dependent.

Item Type: Paper
Uncontrolled Keywords: *Antigens, Viral Blood Cell Adhesion Cell Count Cell Division Cell Line *Cell Transformation, Viral DNA, Viral Floxuridine/pharmacology *Genes, Viral Simian virus 40/*genetics/immunology
Subjects: organism description > virus > SV40
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: January 1978
Date Deposited: 15 Dec 2016 16:21
Last Modified: 15 Dec 2016 16:21
Related URLs:
URI: http://repository.cshl.edu/id/eprint/33296

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