Characterization of a variant of human adenovirus type 2 which multiples efficiently in simian cells

Klessig, D. F., Hassell, J. A. (1978) Characterization of a variant of human adenovirus type 2 which multiples efficiently in simian cells. Journal of Virology, 28 (3). pp. 945-956. ISSN 0022-538X

URL: http://www.ncbi.nlm.nih.gov/pubmed/104054

Abstract

In a previous report (Klessig, J. Virol. 21:1243--1246, 1977), the isolation of a variant (H2hr400) of adenovirus serotype 2 (Ad2) that overcomes the block to multiplication of wild-type Ad2 in simian cells was described. H2hr400 replicates efficiently on both human and simian cells, resulting in virus yields that are comparable to those found when wild-type Ad2 infects permissive, human cells. An extensive comparison of the genome of H2hr400 with that of its parent by restriction endonuclease, electron microscopic, and hybridization analyses failed to detect any differences and excludes the possibility that simian virus 40 sequences, which in certain Ad2-simian virus 40 hybrid viruses (e.g., Ad2+ND1) allow adenovirus to multiply efficiently in simian cells, are present in H2hr400. In contrast to Ad2, H2hr400 can fully express its late genes in both simian and human cells. The mutation has been mapped by a modified marker rescue technique to the segment of the viral genome located between coordinates 59 and 80.

Item Type: Paper
Subjects: organism description > virus > adenovirus
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date Deposited: 13 Dec 2016 18:00
Last Modified: 13 Dec 2016 18:00
Related URLs:
URI: http://repository.cshl.edu/id/eprint/33287

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving