Recognition sequence of restriction endonuclease KpnI from Klebsiella pneumoniae

Tomassini, J., Roychoudhury, R., Wu, R., Roberts, R. J. (November 1978) Recognition sequence of restriction endonuclease KpnI from Klebsiella pneumoniae. Nucleic Acids Res, 5 (11). pp. 4055-64. ISSN 0305-1048 (Print)0305-1048 (Linking)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/82935
DOI: 10.1093/nar/5.11.4055

Abstract

We have determined the recognition sequence of the restriction endonuclease KpnI, previously isolated from Klebsiella pneumoniae. The enzyme cleaves the twofold rotationally symmetric sequence (see book for formula) at the positions indicated by the arrows, producing 3' protruding cohesive ends, four nucleotides in length. The specific cleavage site was unambiguously deduced using both 3' and 5' end analyses of KpnI generated restriction fragments of simian-virus 40 (SV40) DNA (1 site), adenovirus-2 (Ad-2) DNA (8 sites), and a plasmid (pCRI) DNA (2 sites).

Item Type: Paper
Uncontrolled Keywords: Avian Myeloblastosis Virus/enzymology Base Sequence *Dna *DNA Restriction Enzymes Deoxyribonucleases Deoxyribonucleotides/analysis Klebsiella pneumoniae/*enzymology Phosphoric Diester Hydrolases RNA-Directed DNA Polymerase
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > restriction enzyme
CSHL Authors:
Communities: CSHL labs > Roberts lab
Depositing User: Matt Covey
Date: November 1978
Date Deposited: 15 Dec 2016 17:43
Last Modified: 08 Nov 2017 17:13
PMCID: PMC342733
Related URLs:
URI: http://repository.cshl.edu/id/eprint/33285

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