Oncogenic transformation of Drosophila somatic cells induces a functional piRNA pathway

Fagegaltier, D., Falciatori, I., Czech, B., Castel, S., Perrimon, N., Simcox, A., Hannon, G. J. (July 2016) Oncogenic transformation of Drosophila somatic cells induces a functional piRNA pathway. Genes Dev, 30 (14). pp. 1623-35. ISSN 1549-5477 (Electronic)0890-9369 (Linking)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/27474441
DOI: 10.1101/gad.284927.116

Abstract

Germline genes often become re-expressed in soma-derived human cancers as "cancer/testis antigens" (CTAs), and piRNA (PIWI-interacting RNA) pathway proteins are found among CTAs. However, whether and how the piRNA pathway contributes to oncogenesis in human neoplasms remain poorly understood. We found that oncogenic Ras combined with loss of the Hippo tumor suppressor pathway reactivates a primary piRNA pathway in Drosophila somatic cells coincident with oncogenic transformation. In these cells, Piwi becomes loaded with piRNAs derived from annotated generative loci, which are normally restricted to either the germline or the somatic follicle cells. Negating the pathway leads to increases in the expression of a wide variety of transposons and also altered expression of some protein-coding genes. This correlates with a reduction in the proliferation of the transformed cells in culture, suggesting that, at least in this context, the piRNA pathway may play a functional role in cancer.

Item Type: Paper
Uncontrolled Keywords: Hippo Piwi Ras Warts piRNA transposon
Subjects: organism description > animal > insect > Drosophila
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > Piwi protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > piRNA
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > G protein > Ras
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transposons
CSHL Authors:
Communities: CSHL labs > Hannon lab
School of Biological Sciences > Publications
Depositing User: Matt Covey
Date: 15 July 2016
Date Deposited: 04 Aug 2016 19:20
Last Modified: 03 Nov 2017 19:50
PMCID: PMC4973292
Related URLs:
URI: https://repository.cshl.edu/id/eprint/33149

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