Modulation of phototropic responsiveness in Arabidopsis through ubiquitination of phototropin 1 by the CUL3-Ring E3 ubiquitin ligase CRL3(NPH3)

Roberts, D., Pedmale, U. V., Morrow, J., Sachdev, S., Lechner, E., Tang, X., Zheng, N., Hannink, M., Genschik, P., Liscum, E. (October 2011) Modulation of phototropic responsiveness in Arabidopsis through ubiquitination of phototropin 1 by the CUL3-Ring E3 ubiquitin ligase CRL3(NPH3). Plant Cell, 23 (10). pp. 3627-40. ISSN 1040-4651

URL: http://www.ncbi.nlm.nih.gov/pubmed/21990941
DOI: 10.1105/tpc.111.087999

Abstract

Plant phototropism is an adaptive response to changes in light direction, quantity, and quality that results in optimization of photosynthetic light harvesting, as well as water and nutrient acquisition. Though several components of the phototropic signal response pathway have been identified in recent years, including the blue light (BL) receptors phototropin1 (phot1) and phot2, much remains unknown. Here, we show that the phot1-interacting protein NONPHOTOTROPIC HYPOCOTYL3 (NPH3) functions as a substrate adapter in a CULLIN3-based E3 ubiquitin ligase, CRL3(NPH3). Under low-intensity BL, CRL3(NPH3) mediates the mono/multiubiquitination of phot1, likely marking it for clathrin-dependent internalization from the plasma membrane. In high-intensity BL, phot1 is both mono/multi- and polyubiquitinated by CRL3(NPH3), with the latter event targeting phot1 for 26S proteasome-mediated degradation. Polyubiquitination and subsequent degradation of phot1 under high-intensity BL likely represent means of receptor desensitization, while mono/multiubiquitination-stimulated internalization of phot1 may be coupled to BL-induced relocalization of hormone (auxin) transporters.

Item Type: Paper
Uncontrolled Keywords: Animals Arabidopsis/cytology/genetics/*physiology/radiation effects Arabidopsis Proteins/genetics/*metabolism Biological Transport Carrier Proteins/genetics/metabolism Cell Line Cercopithecus aethiops Indoleacetic Acids/metabolism Lepidoptera Light Light Signal Transduction/*physiology/radiation effects Mutation Phosphoproteins/genetics/metabolism Phototropins/genetics/metabolism Phototropism/*physiology/radiation effects Plant Leaves/cytology/genetics/physiology/radiation effects Proteasome Endopeptidase Complex/genetics/metabolism Proteolysis Seedlings/cytology/genetics/physiology/radiation effects Tobacco/genetics/metabolism Ubiquitin-Protein Ligases/genetics/metabolism Ubiquitination/*physiology/radiation effects
Subjects: organism description > plant > Arabidopsis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > plant proteins > phototropins
organism description > plant
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > plant proteins
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein ubiquitination
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > ubiquitin ligase
CSHL Authors:
Communities: CSHL labs > Pedmale lab
Depositing User: Matt Covey
Date: October 2011
Date Deposited: 13 Jun 2016 16:20
Last Modified: 13 Jun 2016 16:20
PMCID: PMC3229139
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32865

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