PHYTOCHROME KINASE SUBSTRATE 1 is a phototropin 1 binding protein required for phototropism

Lariguet, P., Schepens, I., Hodgson, D., Pedmale, U. V., Trevisan, M., Kami, C., de Carbonnel, M., Alonso, J. M., Ecker, J. R., Liscum, E., Fankhauser, C. (June 2006) PHYTOCHROME KINASE SUBSTRATE 1 is a phototropin 1 binding protein required for phototropism. Proc Natl Acad Sci U S A, 103 (26). pp. 10134-9. ISSN 0027-8424 (Print)0027-8424

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URL: http://www.ncbi.nlm.nih.gov/pubmed/16777956
DOI: 10.1073/pnas.0603799103

Abstract

Phototropism, or plant growth in response to unidirectional light, is an adaptive response of crucial importance. Lateral differences in low fluence rates of blue light are detected by phototropin 1 (phot1) in Arabidopsis. Only NONPHOTOTROPIC HYPOCOTYL 3 (NPH3) and root phototropism 2, both belonging to the same family of proteins, have been previously identified as phototropin-interacting signal transducers involved in phototropism. PHYTOCHROME KINASE SUBSTRATE (PKS) 1 and PKS2 are two phytochrome signaling components belonging to a small gene family in Arabidopsis (PKS1-PKS4). The strong enhancement of PKS1 expression by blue light and its light induction in the elongation zone of the hypocotyl prompted us to study the function of this gene family during phototropism. Photobiological experiments show that the PKS proteins are critical for hypocotyl phototropism. Furthermore, PKS1 interacts with phot1 and NPH3 in vivo at the plasma membrane and in vitro, indicating that the PKS proteins may function directly with phot1 and NPH3 to mediate phototropism. The phytochromes are known to influence phototropism but the mechanism involved is still unclear. We show that PKS1 induction by a pulse of blue light is phytochrome A-dependent, suggesting that the PKS proteins may provide a molecular link between these two photoreceptor families.

Item Type: Paper
Uncontrolled Keywords: Arabidopsis/genetics/*physiology Arabidopsis Proteins/analysis/genetics/*metabolism Cell Membrane/chemistry/metabolism Intracellular Signaling Peptides and Proteins/analysis/genetics/*metabolism Membrane Proteins/analysis/genetics/*metabolism Phosphoproteins/analysis/genetics/*metabolism Phototropism/*genetics Phytochrome A/metabolism Protein-Serine-Threonine Kinases/genetics/metabolism
Subjects: organism description > plant > Arabidopsis
organs, tissues, organelles, cell types and functions > tissues types and functions > membranes
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > plant proteins > phototropins
organism description > plant
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > plant proteins
CSHL Authors:
Communities: CSHL labs > Pedmale lab
Depositing User: Matt Covey
Date: 27 June 2006
Date Deposited: 13 Jun 2016 17:03
Last Modified: 09 Nov 2017 16:10
PMCID: PMC1502518
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32859

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