Mapping of Brain Activity by Automated Volume Analysis of Immediate Early Genes

Renier, Nicolas, Adams, Eliza L, Kirst, Christoph, Wu, Zhuhao, Azevedo, Ricardo, Kohl, Johannes, Autry, Anita E, Kadiri, Lolahon, Umadevi Venkataraju, Kannan, Zhou, Yu, Wang, Victoria X, Tang, Cheuk Y, Olsen, Olav, Dulac, Catherine, Osten, Pavel, Tessier-Lavigne, Marc (2016) Mapping of Brain Activity by Automated Volume Analysis of Immediate Early Genes. Cell, 165 (7). pp. 1789-1802. ISSN 0092-8674

URL: http://www.ncbi.nlm.nih.gov/pubmed/27238021
DOI: 10.1016/j.cell.2016.05.007

Abstract

Summary Understanding how neural information is processed in physiological and pathological states would benefit from precise detection, localization, and quantification of the activity of all neurons across the entire brain, which has not, to date, been achieved in the mammalian brain. We introduce a pipeline for high-speed acquisition of brain activity at cellular resolution through profiling immediate early gene expression using immunostaining and light-sheet fluorescence imaging, followed by automated mapping and analysis of activity by an open-source software program we term ClearMap. We validate the pipeline first by analysis of brain regions activated in response to haloperidol. Next, we report new cortical regions downstream of whisker-evoked sensory processing during active exploration. Last, we combine activity mapping with axon tracing to uncover new brain regions differentially activated during parenting behavior. This pipeline is widely applicable to different experimental paradigms, including animal species for which transgenic activity reporters are not readily available.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > immediate early genes
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neuronal circuits
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neuronal circuits
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons > neuronal circuits

Investigative techniques and equipment > Whole Brain Circuit Mapping
CSHL Authors:
Communities: CSHL labs > Osten lab
Depositing User: Matt Covey
Date Deposited: 31 May 2016 20:09
Last Modified: 08 Jul 2016 20:53
PMCID: PMC4912438
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32817

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