Analysis of the sites of integration of viral DNA sequences in rat cells transformed by adenovirus 2 or SV40

Sambrook, J., Greene, R., Stringer, J., Mitchison, T., Hu, S. L., Botchan, M. (1979) Analysis of the sites of integration of viral DNA sequences in rat cells transformed by adenovirus 2 or SV40. Cold Spring Harbor Symposia on Quantitative Biology, 44. pp. 569-584. ISSN 0091-7451

URL: http://www.ncbi.nlm.nih.gov/pubmed/6253158
DOI: 10.1101/SQB.1980.044.01.059

Abstract

All cells transformed by adenovirus type 2 (Ad2) or SV40 carry viral DNA sequences. Often these sequences recombine with those of the host and are then passed on to the cells' descendants like any other part of the cellular genome (Sambrook et al. 1968). While a good deal is known of the number of integrated viral segments present in different cell lines and their general organization (Ketner and Kelly 1976; Botchan et al. 1976; Sambrook et al. 1977), nothing is known of the mechanism by which integration occurs, of the nature of the cellular attachment sites, of the exact locations of the integration sites in the viral genome, or of the mechanism by which viral DNA sequences are expressed. Direct access to the three latter problems has recently become possible with the development of techniques to propagate segments of eukaryotic DNA in prokaryotes

Item Type: Paper
Subjects: organism description > virus > adenovirus
organism description > virus > SV40
organism description > virus > viral transfection
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: 1979
Date Deposited: 06 May 2016 18:51
Last Modified: 06 May 2016 18:51
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32709

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