Unusual base sequence arrangement in phage phi 29 DNA

Ito, J., Roberts, R. J. (January 1979) Unusual base sequence arrangement in phage phi 29 DNA. Gene, 5 (1). pp. 1-7. ISSN 0378-1119 (Print)0378-1119 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/107059
DOI: 10.1016/0378-1119(79)90088-X

Abstract

Susceptibility of Bacillus subtilis phage phi 29 DNA to 34 different restriction endoculceases was determined. Three enzymes, BglI, XbaI and BstEII, were found to cleave phi 29 DNA only once at specific sites. The sites of these single cleavages have been mapped. Thirteen enzymes did not cut phi 29 DNA. phi 29 HindIII DNA fragments inserted into pBR313 plasmid and propagated in Escherichia coli, were resistant to these restriction endonucleases. This result suggests that the insusceptibility is due to the absence of the nucleotide sequences on phi 29 recognized by the enzymes, and not to the presence of modified nucleotides.

Item Type: Paper
Uncontrolled Keywords: Bacillus subtilis Bacteriophages/*genetics Base Sequence DNA Restriction Enzymes/metabolism DNA, Recombinant DNA, Viral/*analysis Escherichia coli/genetics Plasmids
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > bacteriophage

organism description > bacteria > escherichia coli
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > restriction enzyme
CSHL Authors:
Communities: CSHL labs > Roberts lab
Depositing User: Matt Covey
Date: January 1979
Date Deposited: 25 May 2016 19:19
Last Modified: 25 May 2016 19:19
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32693

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