Post-translational modification of actins synthesized in vitro

Garrels, J. I., Hunter, T. (October 1979) Post-translational modification of actins synthesized in vitro. Biochim Biophys Acta, 564 (3). pp. 517-25. ISSN 0006-3002 (Print)0006-3002 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/497226
DOI: 10.1016/0005-2787(79)90041-8

Abstract

It has been shown in two different ways that beta and gamma actins synthesized in vitro are acetylated and that the minor species of actin, delta and epsilon, are nonacetylated forms of beta and gamma actin, respectively. Firstly, additon of acetyl-CoA to the wheat germ system translating poly(A)-containing RNA from unfused rat L6 myoblasts, resulted in an increase in the synthesis of beta and gamma actins at the expense of delta and epsilon actins. Secondly, beta and gamma actins were labeled when synthesized in vitro in the presence of [3H]acetyl-CoA. No label was detectable in delta and epsilon actins. By extrapolation this indicates that beta and gamma actin are acetylated in vivo, probably at the N-terminus. beta and gamma actins synthesized in vivo contain a N tau-methylhistidine residue, but no methylation of beta and gamma actins synthesized in vitro was detectable, using S-[3H]adenosylmethionine as a methyl donor.

Item Type: Paper
Uncontrolled Keywords: Acetyl Coenzyme A/metabolism Acetylation Actins/*biosynthesis Animals Cell Line Methionine/metabolism Methylation Muscles Protein Biosynthesis S-Adenosylmethionine/metabolism
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > actin
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein expression > post-translational modification
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein methylation
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: 25 October 1979
Date Deposited: 23 May 2016 16:16
Last Modified: 23 May 2016 16:16
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32684

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