Changes in protein synthesis during myogenesis in a clonal cell line

Garrels, J. I. (November 1979) Changes in protein synthesis during myogenesis in a clonal cell line. Dev Biol, 73 (1). pp. 134-52. ISSN 0012-1606 (Print)0012-1606 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/527766
DOI: 10.1016/0012-1606(79)90143-X

Abstract

Methods of quantitative 2-dimensional gel electrophoresis were used to study the changes in protein synthesis that occur during myogenic differentiation in the L6 clonal line of rat skeletal muscle cells. Pure populations of myoblasts were obtained by maintaining the cells at subconfluent densities, and virtually pure populations of fused myotubes were obtained by sedimentation at 1 g through a serum gradient. The gel analysis reveals major qualitative differences between myoblasts and myotubes, as well as numerous quantitative changes. Both the .alpha. and the .beta. forms of tropomyosin and the LC2 myosin light chain were increased in rate of synthesis by at least 1000-fold during myogenesis. Other proteins were detectable in myoblasts but were not synthesized at a detectable rate in myotubes. One of these is a form of tropomyosin which comigrates under several electrophoretic conditions with smooth muscle tropomyosin. Another protein, which is repressed in rate of synthesis by at least 1000-fold during myogenesis, appears to be a major form of collagen. Computer analysis has been used to analyze in detail a particular region containing about 300 spots from the 2-dimensional patterns representing protein synthesis in L6 myoblasts, L6 myotubes and a rat nerve cell line. With respect to this set of proteins, the L6 myoblasts and myotubes are no more alike at the level of protein synthesis than are L6 myoblasts and the cells of the nerve line. L6 differentiation involves not only the qualitative switching on and off of major gene products but also the quantitative alteration of synthetic rates of many of the common proteins.

Item Type: Paper
Uncontrolled Keywords: Animals Cell Differentiation Cell Line Clone Cells Collagen/biosynthesis Contractile Proteins/biosynthesis Electrophoresis, Polyacrylamide Gel Muscle Proteins/*biosynthesis Muscles/*cytology Myosins/biosynthesis Rats Tropomyosin/biosynthesis
Subjects: organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell differentiation
Investigative techniques and equipment > assays > gel electrophoresis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > tropomyosin
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: November 1979
Date Deposited: 17 May 2016 15:54
Last Modified: 17 May 2016 15:54
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32683

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