Characterization of the complex between SV40 large T antigen and the 53K host protein in transformed mouse cells.

Crawford, L. V., Lane, D. P., Denhardt, D. T., Harlow, E. E., Nicklin, P. M., Osborn, K., Pim, D. C. (1980) Characterization of the complex between SV40 large T antigen and the 53K host protein in transformed mouse cells. Cold Spring Harbor Symposia on Quantitative Biology, 44. pp. 179-187. ISSN 0091-7451

URL: http://www.ncbi.nlm.nih.gov/pubmed/6253132
DOI: 10.1101/SQB.1980.044.01.021

Abstract

Cells transformed by SV40 express the same functions that appear early after lytic infection. The first polypeptide characterized as both a product of the early region of the viral DNA and characteristic of virus-transformed cells was large T antigen. This protein is the product of the A cistron and has an apparent molecular weight of about 94,000 (Rundell et al. 1977). The second polypeptide, small T antigen, was initially detected by in vitro protein synthesis (Greenblatt et al. 1976; Prives et al. 1977). It has a molecular weight of about 20,000 after alkylation (Crawford and O'Farrell 1979), consistent with the estimate of 20,503 for its molecular weight based on the DNA sequence (Fiers et al. 1978; Reddy et al. 1978). Large and small T antigens have common N-terminal sequences (Paucha et al. 1978) and share antigenic determinants (Lane and Robbins 1978).

Item Type: Paper
Subjects: organism description > virus > SV40
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: 1980
Date Deposited: 02 Jun 2016 16:20
Last Modified: 02 Jun 2016 16:20
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32674

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