Isolation and characterization of mutations in the structural gene for protease III (ptr)

Cheng, Y. S., Zipser, D., Cheng, C. Y., Rolseth, S. J. (October 1979) Isolation and characterization of mutations in the structural gene for protease III (ptr). J Bacteriol, 140 (1). pp. 125-30. ISSN 0021-9193 (Print)0021-9193 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/387716

Abstract

Escherichia coli mutants defective in protease III were isolated by enzyme assays of heavily mutagenized colones. One mutant produced thermolabile enzyme, and it is presumed to have a mutation in the structural gene of protease III. Two other mutants mapping at the same site had less than 5% of the wild-type protease III level. The genetic locus of these mutations, designated ptr, was located at approximately 60 min on the E. coli linkage map based on its high frequency (70%) of contransduction by P1 with argA. Strains with less than 5% of the wild-type protease III activity grew normally and degraded nonsense fragments of beta-galactosidase at wild-type rates.

Item Type: Paper
Uncontrolled Keywords: Chromosome Mapping Chromosomes, Bacterial Escherichia coli/enzymology/*genetics *Genes Mutation Peptide Hydrolases/*genetics/metabolism
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome

organism description > bacteria > escherichia coli
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: October 1979
Date Deposited: 26 May 2016 16:53
Last Modified: 26 May 2016 16:53
PMCID: PMC216787
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32670

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