Cheng, Y. S., Zipser, D., Cheng, C. Y., Rolseth, S. J. (October 1979) Isolation and characterization of mutations in the structural gene for protease III (ptr). J Bacteriol, 140 (1). pp. 125-30. ISSN 0021-9193 (Print)0021-9193 (Linking)
Abstract
Escherichia coli mutants defective in protease III were isolated by enzyme assays of heavily mutagenized colones. One mutant produced thermolabile enzyme, and it is presumed to have a mutation in the structural gene of protease III. Two other mutants mapping at the same site had less than 5% of the wild-type protease III level. The genetic locus of these mutations, designated ptr, was located at approximately 60 min on the E. coli linkage map based on its high frequency (70%) of contransduction by P1 with argA. Strains with less than 5% of the wild-type protease III activity grew normally and degraded nonsense fragments of beta-galactosidase at wild-type rates.
| Item Type: | Paper |
|---|---|
| Uncontrolled Keywords: | Chromosome Mapping Chromosomes, Bacterial Escherichia coli/enzymology/*genetics *Genes Mutation Peptide Hydrolases/*genetics/metabolism |
| Subjects: | bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome organism description > bacteria > escherichia coli bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function |
| CSHL Authors: | |
| Communities: | CSHL labs |
| Depositing User: | Matt Covey |
| Date: | October 1979 |
| Date Deposited: | 26 May 2016 16:53 |
| Last Modified: | 26 May 2016 16:53 |
| PMCID: | PMC216787 |
| Related URLs: | |
| URI: | https://repository.cshl.edu/id/eprint/32670 |
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