Identification and mapping of the transcriptional and translational products of the yeast plasmid, 2mu circle

Broach, J. R., Atkins, J. F., McGill, C., Chow, L. (April 1979) Identification and mapping of the transcriptional and translational products of the yeast plasmid, 2mu circle. Cell, 16 (4). pp. 827-39. ISSN 0092-8674 (Print)0092-8674 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/378400
DOI: 10.1016/0092-8674(79)90098-9

Abstract

We have identified two major and approximately ten minor poly(A)-containing RNA species in S. cerevisiae which arise from in vivo transcription of the yeast plasmid, known as 2mu circle. The two major species, which are 1325 and 1275 bases in length, are transcribed from the two unique halves of the plasmid and extend into the inverted repeat sequences which separate the unique regions. The map positions of the minor transcripts, which range in length from 350 to 2600 bases, indicate that except for a small region of the genome in which no transcription is observed, both strands of the entire 2mu circle genome are transcribed. We also present evidence demonstrating that RNA transcribed from 2mu circular DNA is used to program the synthesis of specific proteins in yeast: that is, yeast RNA complementary to 2mu circle DNA can be translated in vitro to produce specific polypeptides of substantial size. Finally, the pattern of transcription of 2mu circle suggests the possibility that messenger RNA species are derived by cleavage of larger transcripts, and in addition, that the intramolecular recombination of 2mu circle which occurs in yeast functions as a genetic switch to allow separate expression of two sets of genes on the 2mu circle genome.

Item Type: Paper
Uncontrolled Keywords: Binding Sites Chromosome Mapping DNA-Directed RNA Polymerases/metabolism Fungal Proteins/genetics Nucleic Acid Hybridization *Plasmids Protein Biosynthesis RNA, Messenger/genetics Saccharomyces cerevisiae/*genetics Transcription, Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > plasmid
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > translation
organism description > yeast
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: April 1979
Date Deposited: 09 Aug 2016 15:40
Last Modified: 09 Aug 2016 15:40
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32662

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