Galactose regulation in Saccharomyces cerevisiae. The enzymes encoded by the GAL7, 10, 1 cluster are co-ordinately controlled and separately translated

Broach, J. R. (1979) Galactose regulation in Saccharomyces cerevisiae. The enzymes encoded by the GAL7, 10, 1 cluster are co-ordinately controlled and separately translated. J Mol Biol, 131 (1). pp. 41-53. ISSN 0022-2836 (Print)0022-2836 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/385888
DOI: 10.1016/0022-2836(79)90300-0

Abstract

The enzymes for galactose metabolism in Saccharomyces cerevisiae are encoded by three tightly linked genes. Data presented in this paper show that, in contrast to enzymes encoded by other gene clusters in yeast, these three enzymes are translated as separate polypeptides. First, two of the enzymes encoded by the cluster, galactokinase and uridylyl transferase. purified to near homogeneity, are separate polypeptides. Second, no precursor polypeptide-containing sequences common to both these enzymes is detectable in extracts from galactose-induced yeast cells. Third, no partial or absolute polarity of expression of the enzymes is observed in strains containing nonsense mutations in any of the genes of the cluster. Expression of the three galactose metabolic enzymes is co-ordinate, both during induction and during steady-state synthesis. This is true both for wild-type yeast strains and for strains carrying the long-term galactose adaptation mutation, gal3. In GAL3+ strains mutations within the galactose gene cluster have no effect on this co-ordinate expression. However, in gal3− strains, mutations in any of the genes of the cluster completely eliminate expression of the other two genes. These results suggest that the GAL3 gene product is responsible for inducer synthesis and that the actual inducer is an intermediate in galactose metabolism.

Item Type: Paper
Uncontrolled Keywords: Electrophoresis, Polyacrylamide Gel Enzyme Induction Enzyme Precursors Galactokinase/genetics/metabolism Galactose/*genetics/metabolism Genes *Genes, Regulator Mutation Protein Biosynthesis Saccharomyces cerevisiae/*genetics/metabolism UDPglucose-Hexose-1-Phosphate Uridylyltransferase/genetics/metabolism
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene regulation

organism description > yeast
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date Deposited: 09 Aug 2016 15:15
Last Modified: 09 Aug 2016 15:15
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32661

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