Studies on simian virus 40 excision from cellular chromosomes

Botchan, M., Topp, W., Sambrook, J. (1979) Studies on simian virus 40 excision from cellular chromosomes. Cold Spring Harb Symp Quant Biol, 43. pp. 709-19. ISSN 0091-7451 (Print)0091-7451 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/226315
DOI: 10.1101/SQB.1979.043.01.079

Abstract

Rodent cells in culture which do not support SV40 replication can be stably transformed by the virus. The resulting cells, which invariably harbor integrated viral DNA sequences (Sambrook et al. 1968; Hirai et al. 1971; Gelb et al. 1971), often can be manipulated to become infectious centers of viral production by fusion with permissive simian cells (Gerber 1966; Watkins and Dulbecco 1967; Koprowski et al. 1967). The source of the progeny viral DNA molecules is the integrated copy (see Discussion). This is remarkable in view of the fact that previous work from our laboratory and others has shown that integrated SV40 DNA can be attached to chromosomal DNA at various positions on the viral genome and also that multiple sites in cellular DNA are available for this viral insertion (Botchan et al. 1976; Ketner and Kelly 1976; Kucherlapati et al. 1978).

Item Type: Paper
Uncontrolled Keywords: Animals *Cell Transformation, Viral Cells, Cultured Chromosomes/metabolism DNA Replication DNA, Viral/*metabolism *Genes, Viral Haplorhini Recombination, Genetic Replicon Simian virus 40/*genetics Viral Proteins/metabolism *Virus Replication
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome

organism description > virus > SV40
organism description > virus
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: 1979
Date Deposited: 09 Aug 2016 14:53
Last Modified: 09 Aug 2016 14:53
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32660

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