The nucleotide sequences at the termini of adenovirus-2 DNA

Arrand, J. R., Roberts, R. J. (1979) The nucleotide sequences at the termini of adenovirus-2 DNA. Journal of Molecular Biology, 128 (4). pp. 577-594. ISSN 0022-2836

URL: http://www.ncbi.nlm.nih.gov/pubmed/318164
DOI: 10.1016/0022-2836(79)90294-8

Abstract

The nucleotide sequence of the first 156 residues from the left end and the first 134 residues from the right end of adenovirus-2 DNA have been determined by direct DNA sequencing techniques. The inverted terminal repetition is 102 nucleotide pairs long. The 5′-ends of the intact DNA are resistant to the action of T4 polynucleotide kinase and the 5′ → 3′ exonucleases from phages lambda and T7. This resistance is most likely due to the covalent attachment of the 5′-terminal C residue to the terminal protein. No significant self-complementarity exists within the inverted terminal repetition, making terminal initiation of DNA replication via a self-priming mechanism unlikely. However, the terminal A + T-rich region followed immediately by a very G + C-rich region is consistent with other schemes for adenovirus-2 DNA replication. The left end of adenovirus-2 DNA contains extensive sequence repetition.

Item Type: Paper
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > DNA replication
organism description > virus > adenovirus
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > restriction enzyme
CSHL Authors:
Communities: CSHL labs > Roberts lab
Depositing User: Matt Covey
Date: 1979
Date Deposited: 10 Aug 2016 18:33
Last Modified: 10 Aug 2016 18:33
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32651

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