Genome mapping by nonrandom anchoring: a discrete theoretical analysis

Zhang, M. Q., Marr, T. G. (January 1993) Genome mapping by nonrandom anchoring: a discrete theoretical analysis. Proc Natl Acad Sci U S A, 90 (2). pp. 600-4. ISSN 0027-8424 (Print)0027-8424 (Linking)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/8421694
DOI: 10.1073/pnas.90.2.600

Abstract

As part of our effort to construct a physical map of the genome of the fission yeast Schizosaccharomyces pombe, we have made theoretical predictions for the progress expected, as measured by the expected length fraction of island coverage and by the expected properties of the anchored islands such as the number and the size of islands. Our experimental strategy is to construct a random clone library and screen the library for clones having unique sequence at both ends. This scheme is essentially the same as the clone-limited double sequence-tagged-site selection scheme which was used in a computer simulation by Palazzolo et al. [Palazzolo, M. J., Sawyer, S. A., Martin, C. H., Smoller, D. A. & Hartl, D. L. (1991) Proc. Natl. Acad. Sci. USA 88, 8034-8038]. Both simulation and ongoing experiments in our laboratory have shown that the nonrandom anchoring method is far superior to random anchoring. In this paper, we propose a theoretical model to explain the simulated data and the experimental data.

Item Type: Paper
Uncontrolled Keywords: Chromosome Mapping/ methods Computer Simulation Gene Library Genome, Fungal Models, Genetic Schizosaccharomyces/ genetics Sequence Tagged Sites
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > genomes
CSHL Authors:
Communities: CSHL labs > Zhang lab
Depositing User: Matt Covey
Date: 15 January 1993
Date Deposited: 08 Apr 2016 20:12
Last Modified: 09 Nov 2017 20:05
PMCID: PMC45711
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32593

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