Preferential expression in mushroom bodies of the catalytic subunit of protein kinase A and its role in learning and memory

Skoulakis, E. M., Kalderon, D., Davis, R. L. (August 1993) Preferential expression in mushroom bodies of the catalytic subunit of protein kinase A and its role in learning and memory. Neuron, 11 (2). pp. 197-208. ISSN 0896-6273 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/8352940
DOI: 10.1016/0896-6273(93)90178-T

Abstract

Involvement of the cAMP cascade in Drosophila learning and memory is suggested by the aberrant behavioral phenotypes of the mutants dunce (cAMP phosphodiesterase) and rutabaga (adenylyl cyclase). Line DCO581, isolated via an enhancer detector screen for genes preferentially expressed in the mushroom bodies, contains a transposon in the first exon of the catalytic subunit gene (DCO) of protein kinase A (PKA). RNA in situ hybridization and immunohistochemistry show that DCO is preferentially expressed in the mushroom bodies. The DCO581 insertion and an independently isolated hypomorphic allele (DCOB10) each produce homozygous lethality and a 40% decrease in PKA activity in heterozygotes. This decrease has mild effects on learning but no effect on memory. However, the 80% reduction in activity obtained by constructing heteroallelic yet viable DCO581/DCOB10 animals results in a dramatic learning and memory deficit. These results suggest that PKA plays a crucial role in the cAMP cascade in mushroom bodies to mediate learning and memory processes.

Item Type: Paper
Uncontrolled Keywords: Animals Base Sequence Behavior, Animal/physiology Brain/ metabolism Catalysis Drosophila Gene Expression Learning/ physiology Memory/ physiology Molecular Sequence Data Mutagenesis, Insertional Protein Kinases/chemistry/genetics/ physiology Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S.
Subjects: organism description > animal > insect > Drosophila
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
organism description > animal behavior > learning
organism description > animal behavior > memory
organs, tissues, organelles, cell types and functions > tissues types and functions > mushroom body
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: August 1993
Date Deposited: 12 Apr 2016 19:57
Last Modified: 12 Apr 2016 19:57
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32580

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving