Characterization of the inducer of short transcripts, a human immunodeficiency virus type 1 transcriptional element that activates the synthesis of short RNAs

Sheldon, M., Ratnasabapathy, R., Hernandez, N. (1993) Characterization of the inducer of short transcripts, a human immunodeficiency virus type 1 transcriptional element that activates the synthesis of short RNAs. Mol Cell Biol, 13 (2). pp. 1251-63. ISSN 0270-7306 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/8423790
DOI: 10.1128/MCB.13.2.1251

Abstract

The inducer of short transcripts, or IST, is an unusual transcriptional element located downstream of the human immunodeficiency virus type 1 (HIV-1) promoter. IST activates HIV-1 transcription, but the resulting RNAs are short and end at approximately position +59. IST, therefore, appears to promote the formation of transcription complexes that are unable to elongate efficiently. This activity contrasts with that of TAR, the target for Tat trans-activation, which upon binding of the viral protein Tat promotes the formation of transcription complexes capable of efficient elongation through the entire viral genome. We have localized and characterized the IST element. Our results indicate that IST is located mainly between positions -5 and +26, although the sequences from positions +40 to +59 also contribute to IST activity. Unlike TAR, which is an RNA element, IST appears to be a DNA element. Thus, the HIV-1 R region is a complex regulatory region with RNA and DNA elements that promote the formation of transcription complexes with different elongation properties.

Item Type: Paper
Uncontrolled Keywords: Base Sequence Cell Line DNA, Viral/ genetics HIV-1/ genetics Hela Cells Humans Molecular Sequence Data Mutagenesis, Site-Directed Nucleic Acid Conformation Promoter Regions (Genetics) RNA, Viral/ biosynthesis Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Transcription, Genetic
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
diseases & disorders > viral diseases > HIV
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations > mutagenesis
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > sRNA
CSHL Authors:
Communities: CSHL labs > Hernandez lab
Depositing User: Matt Covey
Date Deposited: 13 Apr 2016 15:32
Last Modified: 13 Apr 2016 15:32
PMCID: PMC359010
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32561

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