Proliferating cell nuclear antigen and p21 are components of multiple cell cycle kinase complexes

Zhang, H., Xiong, Y., Beach, D. (September 1993) Proliferating cell nuclear antigen and p21 are components of multiple cell cycle kinase complexes. Mol Biol Cell, 4 (9). pp. 897-906. ISSN 1059-1524 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/7903056
DOI: 10.1091/mbc.4.9.897

Abstract

We have recently shown that two proteins, proliferating cell nuclear antigen (PCNA) and p21, are associated with cyclin D. Here we show that PCNA and p21 are common components of a wide variety of cyclin/cyclin-dependent kinase complexes in nontransformed cells. These include kinase complexes containing cyclin A, cyclin B, and cyclin D, associated either with CDC2, CDK2, CDK4, or CDK5. We show that PCNA and p21 form separate quaternary complex with each cyclin/CDK and that these quaternary complexes contain a substantial, if not major, fraction of the cell cycle kinases in asynchronously growing cells. These results suggest that PCNA and p21 may perform a common function for all these kinases.

Item Type: Paper
Uncontrolled Keywords: Animals Autoantigens/analysis Autoradiography Blotting, Western CDC2 Protein Kinase/biosynthesis/ chemistry/isolation & purification Cell Line Centrifugation, Density Gradient Cyclin-Dependent Kinase Inhibitor p21 Cyclins/ analysis/ metabolism Electrophoresis, Polyacrylamide Gel Hela Cells Humans Male Methionine/metabolism Molecular Weight Nuclear Proteins/ analysis Proliferating Cell Nuclear Antigen Protein Kinases/biosynthesis/ chemistry/isolation & purification Research Support, U.S. Gov't, P.H.S. Sulfur Radioisotopes
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > Cyclins
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > cdc2
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > Proliferating Cell Nuclear Antigen
CSHL Authors:
Communities: CSHL labs > Beach lab
Depositing User: Matt Covey
Date: September 1993
Date Deposited: 13 Apr 2016 15:05
Last Modified: 13 Apr 2016 15:05
PMCID: PMC275720
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32559

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