Regulation of neuropeptide expression in cultured cerebral cortical neurons by brain-derived neurotrophic factor

Nawa, H., Bessho, Y., Carnahan, J., Nakanishi, S., Mizuno, K. (1993) Regulation of neuropeptide expression in cultured cerebral cortical neurons by brain-derived neurotrophic factor. J Neurochem, 60 (2). pp. 772-5. ISSN 0022-3042 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/8093484
DOI: 10.1111/j.1471-4159.1993.tb03216.x

Abstract

The neuropeptide-inducing activity of neurotrophic factors was tested in cultured cerebral cortical neurons. Brain-derived neurotrophic factor (BDNF) specifically increased contents of the neuropeptides somatostatin (SOM) and neuropeptide Y (NPY), but its effect on contents of cholecystokinin octapeptide and GABA was much less significant. The maximal induction of NPY content (15-fold increase) was achieved by 20 ng/ml of BDNF. These changes were also reproduced at the mRNA level. In contrast, neurotrophin-3 was much less potent at increasing NPY and SOM contents, and nerve growth factor had no effect on them. The expression of mRNA for NPY and SOM was fully dependent on the presence of BDNF in culture but irrelevant to the survival-promoting activity of BDNF, which has been reported previously. Most of the NPY immunoreactivity induced by BDNF was colocalized with glutamate decarboxylase immunoreactivity in cultured cortical neurons. These results suggest that BDNF regulates the peptidergic expression of GABAergic neurons in the cerebral cortex.

Item Type: Paper
Uncontrolled Keywords: Animals Blotting, Northern Brain-Derived Neurotrophic Factor Cells, Cultured Cerebral Cortex/ metabolism DNA Probes Dizocilpine Maleate/pharmacology Embryo Glutamate Decarboxylase/metabolism Kinetics Nerve Growth Factors/ pharmacology Nerve Tissue Proteins/ pharmacology Neurons/drug effects/ metabolism Neuropeptide Y/ biosynthesis/genetics RNA, Messenger/metabolism Rats Research Support, Non-U.S. Gov't Sincalide/ metabolism Somatostatin/biosynthesis gamma-Aminobutyric Acid/ metabolism
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > brain derived neurotrophic factor
Investigative techniques and equipment > cell culture
organs, tissues, organelles, cell types and functions > tissues types and functions > cerebral cortex
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mRNA
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
organs, tissues, organelles, cell types and functions > cell types and functions > cell types > neurons
CSHL Authors:
Communities: CSHL labs > Yin lab
Depositing User: Matt Covey
Date Deposited: 15 Apr 2016 20:00
Last Modified: 15 Apr 2016 20:00
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32525

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