Chromosomal Structure and Expression of the Human Otf1 Locus Encoding the Oct-1 Protein

Sturm, R. A., Cassady, J. L., Das, G., Romo, A., Evans, G. A. (May 1993) Chromosomal Structure and Expression of the Human Otf1 Locus Encoding the Oct-1 Protein. Genomics, 16 (2). pp. 333-341. ISSN 0888-7543

URL: http://www.ncbi.nlm.nih.gov/pubmed/8314572
DOI: 10.1006/geno.1993.1194

Abstract

The genomic structure of the POU domain containing oct-1 gene (OTF1 locus) coding region has been determined using human DNA recombinant bacteriophage and a yeast artificial chromosome clone. The gene is encoded by 16 exons spanning over 150 kb, and the Oct-1 protein reading frame has been extended to 766 amino acids. The exonic structure has been compared to the mouse Oct-2 protein and reveals a conservation of exon-intron boundaries as well as protein sequence similarity. To provide insight into Oct-1 control of transcriptional regulation during the cell-cycle the expression of the oct-1 gene was examined during cellular DNA replication and shows that the steady-state level of the oct-1 mRNA is not S-phase regulated.

Item Type: Paper
Uncontrolled Keywords: DNA-BINDING ACTIVITY TRANSCRIPTION FACTOR POU-DOMAIN CELL-CYCLE GENE CONTAINS VECTORS CDNA BOX PHOSPHORYLATION
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome

bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > exons
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > gene expression
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > histone
CSHL Authors:
Communities: CSHL labs > Herr lab
Depositing User: Matt Covey
Date: May 1993
Date Deposited: 19 Apr 2016 16:11
Last Modified: 19 Apr 2016 16:11
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32519

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