DNA bending and orientation-dependent function of YY1 in the c-fos promoter

Natesan, S., Gilman, M. Z. (December 1993) DNA bending and orientation-dependent function of YY1 in the c-fos promoter. Genes Dev, 7 (12B). pp. 2497-509. ISSN 0890-9369 (Print)

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URL: http://www.ncbi.nlm.nih.gov/pubmed/8276234
DOI: 10.1101/gad.7.12b.2497

Abstract

The assembly of multicomponent complexes at promoters, enhancers, and silencers likely entails perturbations in the path of the DNA helix. We present evidence that YY1, a ubiquitously expressed DNA-binding protein, regulates the activity of the c-fos promoter primarily through an effect on DNA structure. YY1 binds to and induces a phased DNA bend at three sites in this promoter. By use of a truncated c-fos promoter activity containing a single functional YY1 site, we show that YY1 represses promoter activity but that repression does not appear to be an intrinsic property of the protein in this context. Moreover, when the orientation of the YY1 site is reversed, YY1 activates the same promoter. Repression by YY1 is also alleviated by changing the relative phasing of factor-binding sites on either side of YY1. We conclude that the principal function of YY1 in this promoter is to bend DNA to regulate contact between other proteins. Thus, YY1 represents a new class of transcription factors that influences promoter function by affecting promoter structure rather than by directly contacting the transcriptional machinery. We provide evidence that the product of the male sex determination gene SRY may also belong to this class of structural factors.

Item Type: Paper
Uncontrolled Keywords: Animals Base Sequence Binding Sites Cyclic AMP Response Element-Binding Protein/metabolism DNA/ chemistry/metabolism DNA-Binding Proteins/metabolism/ physiology Drosophila/cytology Erythroid-Specific DNA-Binding Factors Genes, fos/ genetics Hela Cells Humans Mice Molecular Sequence Data Nucleic Acid Conformation Promoter Regions (Genetics)/ genetics Research Support, U.S. Gov't, P.H.S. TATA Box Transcription Factors/metabolism/ physiology YY1 Transcription Factor
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > transcription
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > genes, structure and function > genes: types > c-fos
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > transcription factor
CSHL Authors:
Communities: CSHL labs
Depositing User: Matt Covey
Date: December 1993
Date Deposited: 19 Apr 2016 15:04
Last Modified: 03 Nov 2017 20:31
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32515

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