Characterization of the fission yeast mcs2 cyclin and its associated protein kinase activity

Molz, L., Beach, D. (April 1993) Characterization of the fission yeast mcs2 cyclin and its associated protein kinase activity. Embo J, 12 (4). pp. 1723-32. ISSN 0261-4189 (Print)

URL: http://www.ncbi.nlm.nih.gov/pubmed/8467814

Abstract

We have previously described the isolation of mcs2-75, a mutation obtained as an allele-specific suppressor of a dominant allele of cdc2. mcs2 was cloned and determined to be an essential gene, the product of which shares homology with the cyclin family of proteins. In contrast to the behavior of some, but not all cyclins, the mcs2 protein is constant in its abundance and localization throughout the cell cycle. A kinase activity that co-precipitates with mcs2 can be detected when myelin basic protein (MBP) is provided as an exogenous substrate. This kinase activity is constant throughout the cell cycle. mcs2 does not appear to associate with the cdc2 protein kinase or an antigenically related kinase. Finally, a protein kinase termed csk1 (cyclin suppressing kinase) was isolated as a high copy suppressor of an mcs2 mutation. csk1 is not essential, however, the level of kinase activity that co-precipitates with mcs2 is reduced approximately 3-fold in strains harboring a csk1 null allele. Therefore, csk1 may encode a protein kinase physically associated with mcs2 or alternatively may function as an upstream activator of the mcs2-associated kinase.

Item Type: Paper
Uncontrolled Keywords: Amino Acid Sequence Base Sequence Cell Cycle Cell Nucleus/metabolism Cloning, Molecular Comparative Study Cyclins/ physiology Genes, Fungal Genes, Suppressor Molecular Sequence Data Nuclear Proteins/ genetics Protein Kinases/ physiology Research Support, Non-U.S. Gov't Research Support, U.S. Gov't, P.H.S. Restriction Mapping Schizosaccharomyces/ enzymology/genetics Sequence Alignment
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > Cyclins
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > enzymes > kinase
organism description > yeast
CSHL Authors:
Communities: CSHL labs > Beach lab
Depositing User: Matt Covey
Date: April 1993
Date Deposited: 19 Apr 2016 19:39
Last Modified: 19 Apr 2016 19:39
PMCID: PMC41338
URI: http://repository.cshl.edu/id/eprint/32505

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