A 13-Kb Resolution Cosmid Map of the 14-Mb Fission Yeast Genome by Nonrandom Sequence-Tagged Site Mapping

Mizukami, T., Chang, W. I., Garkavtsev, I., Kaplan, N., Lombardi, D., Matsumoto, T., Niwa, O., Kounosu, A., Yanagida, M., Marr, T. G., Beach, D. (April 1993) A 13-Kb Resolution Cosmid Map of the 14-Mb Fission Yeast Genome by Nonrandom Sequence-Tagged Site Mapping. Cell, 73 (1). pp. 121-132. ISSN 0092-8674

URL: http://www.ncbi.nlm.nih.gov/pubmed/8462095
DOI: 10.1016/0092-8674(93)90165-M

Abstract

We present the application of a nonrandom sequence-tagged site (STS) content detection method in mapping an entire genome, that of fission yeast. The novelty of our strategy is in the use of STS probes made from both ends of cosmid clones, selected on the basis of ''sample without replacement'' (only library clones that show no previous positive hybridization are selected and made into probes). We developed powerful techniques, based on consistency analysis, for error detection and contig assembly. In addition, we probed our library with genetically mapped markers and NotI or SfiI linking clones, thereby anchoring contigs onto chromosomes. Our map contains more than 1000 sites, including genes (most were previously unmapped), occurrences of known repetitive elements, and NotI-SfiI restriction sites.

Item Type: Paper
Uncontrolled Keywords: SCHIZOSACCHAROMYCES-POMBE GENE IDENTIFICATION INTEGRATION CHROMOSOMES FRAGMENTS
Subjects: bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosome
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > chromosomes, structure and function > chromosome

organism description > yeast
CSHL Authors:
Communities: CSHL labs > Beach lab
CSHL labs > McCombie lab
Depositing User: Matt Covey
Date: April 1993
Date Deposited: 21 Apr 2016 14:46
Last Modified: 21 Apr 2016 14:46
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32494

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