A versatile microtiter assay for the universal cdc2 cell cycle regulator

Ducommun, B., Beach, D. (May 1990) A versatile microtiter assay for the universal cdc2 cell cycle regulator. Anal Biochem, 187 (1). pp. 94-7. ISSN 0003-2697 (Print)0003-2697 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/2164794
DOI: 10.1016/0003-2697(90)90422-6

Abstract

A microassay for p34cdc2 based on the high affinity association between cdc2 and Schizosaccharomyces pombe p13suc1 has been developed. p13 purified from Escherichia coli was immobilized on microtiter plates and cellular lysate was incubated in the wells to allow the binding of cdc2 and its associated proteins. p34cdc2 was assayed either as a histone kinase or by immunological methods. The method was optimized for S. pombe cell extracts but can also be applied to other organisms such as Xenopus oocytes or HeLa cells. This rapid assay allows the specific determination of p34cdc2 histone H1 kinase activity in a very large number of samples.

Item Type: Paper
Uncontrolled Keywords: Animals CDC2 Protein Kinase Cell Cycle/*physiology *Cell Cycle Proteins Cell Line *Fungal Proteins HeLa Cells Humans Immunoenzyme Techniques Oocytes Phosphoproteins/*analysis/metabolism Protamine Kinase/metabolism Saccharomyces *Schizosaccharomyces pombe Proteins Xenopus
Subjects: Investigative techniques and equipment > assays
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > cdc2
organs, tissues, organelles, cell types and functions > cell types and functions > cell functions > cell cycle
CSHL Authors:
Communities: CSHL labs > Beach lab
Depositing User: Matt Covey
Date: 15 May 1990
Date Deposited: 08 Feb 2016 16:26
Last Modified: 08 Feb 2016 16:26
Related URLs:
URI: http://repository.cshl.edu/id/eprint/32352

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