The regions of the retinoblastoma protein needed for binding to adenovirus E1A or SV40 large T antigen are common sites for mutations

Hu, Q. J., Dyson, N., Harlow, E. (April 1990) The regions of the retinoblastoma protein needed for binding to adenovirus E1A or SV40 large T antigen are common sites for mutations. EMBO J, 9 (4). pp. 1147-55. ISSN 0261-4189 (Print)0261-4189 (Linking)

URL: http://www.ncbi.nlm.nih.gov/pubmed/2138977

Abstract

The protein product of the retinoblastoma (RB) gene is thought to function in a pathway that restricts cell proliferation. Recently, transforming proteins from three different classes of DNA tumor viruses have been shown to form complexes with the RB protein. Genetic studies suggest that these interactions with the RB protein are important steps in transformation by these viruses. In order to understand better the function of the RB-viral oncoprotein complexes, we have mapped the regions of the RB protein that are necessary for these associations. Two non-contiguous regions of RB were found to be essential for complex formation with adenovirus E1A or SV40 large T antigen. These two regions are found between amino acids 393 and 572 and 646 and 772. Interestingly, these binding sites on RB overlap with the positions of naturally occurring, inactivating mutations of the RB gene. These results strongly suggest that these viral oncoproteins are targeting a protein domain that is an important site in the normal function of the RB protein.

Item Type: Paper
Uncontrolled Keywords: Adenovirus Early Proteins Base Sequence Binding Sites Chromosome Deletion DNA-Binding Proteins/metabolism Eye Neoplasms/genetics Humans Molecular Sequence Data *Mutation Oligonucleotide Probes Oncogene Proteins, Viral/*metabolism Phosphoproteins/*genetics/metabolism Polymerase Chain Reaction Protein Binding Protein Biosynthesis Retinoblastoma/genetics Retinoblastoma Protein Suppression, Genetic
Subjects: organism description > virus > adenovirus
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > protein structure, function, modification > protein types > DNA binding protein
bioinformatics > genomics and proteomics > genetics & nucleic acid processing > DNA, RNA structure, function, modification > mutations
diseases & disorders > cancer > cancer types > retinoblastoma
CSHL Authors:
Communities: CSHL labs > Stillman lab
Depositing User: Matt Covey
Date: April 1990
Date Deposited: 12 Feb 2016 16:19
Last Modified: 12 Feb 2016 16:19
PMCID: PMC551790
URI: http://repository.cshl.edu/id/eprint/32321

Actions (login required)

Administrator's edit/view item Administrator's edit/view item
CSHL HomeAbout CSHLResearchEducationNews & FeaturesCampus & Public EventsCareersGiving